The impact of different drugs on relaxation or contraction of the endothelium or vascular smooth muscles can be investigated using isolated tissue bath protocols.

This application note includes:

• - An introduction about the crucial role of the endothelium
  
• - A focus on recent studies where tissue baths were used to investigate cardiovascular diseases: Atherosclerosis, hypertension, heart failure, diabetes and metabolic syndrome
  
• - A protocol explaining how to set an experiment to study rodents aorta rings on tissue bath
  
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